Micropropagation of Leucojum aestivum L. in bioraector RITA®
Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS
Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN
Embryogenic callus of Leucojum aestivum was transferred to solid and liquid media in bioreactor RITA® vessels. Callus growth on the liquid medium enriched with 2 and 5 qM Picloram and 0.5 qM BA was characterized by a higher multiplication index. The greatest number of somatic embryos was observed on the callus multiplied in bioreactor using 5-minute flushing with the medium. Globular embryos developed into torpedo-stage embryos and plantlets under the influence of NAA (0.5 qM) and zeatin (5 qM). Liquid medium promoted the development of L aestivum somatic embryos and plantlets.
Biotechnologia, vol.89, 2 (2010)-.
oai:rcin.org.pl:71618 ; IChB B-84
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Oct 2, 2020
May 27, 2019
854
https://rcin.org.pl./publication/93892
Edition name | Date |
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Mikrorozmnażanie Leucolum aesHvum L. w bioreaktorze RITA® | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Ptak, Agata Cierniak, Oliwia
Nowak, Jacek K.