Subtitle:

Topophysis in adventitious shoots regeneration in vitro in chrysanthemum

Contributor:

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Publisher:

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

Description:

prace eksperymentalne

Abstract:

Success of breeding programmes using adventitious shoots technique often depends on the regeneration efficiency. The aim of this study was to investigate the influence of topophysical position of explants on the efficiency of adventitious shoots regeneratio. Uniform, single shoots of chrysanthemum ‘Satinbleu’ propagated in vitro were divided equally into three topophysical zones: distal, central and proximal. Two leaves and two internodes were isolated from each zone. The explants were cultured for 12 weeks on MS medium supplemented with 0.6 mg 1 ' BAP and 2.0 mg I ' 1AA. Duo to the highest adventitious shoots regeneration capability and the earliest formation of the shoots, the most suitable explants for breeding programmes for chrysanthemum are internodes excised from distal part of in vitro plantlet.

Relation:

Biotechnologia, vol.89, 2 (2010)-.

Volume:

89

Issue:

2

Start page:

89

End page:

95

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

oai:rcin.org.pl:71588 ; IChB B-84

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open