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Utility of starch raw materials for biosynthesis of glucose oxidase by Aspergillus niger
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Potato starch and its hydrolyzates were used to substitute glucose carbon sources to synthesize glucose oxidase (GOD) by free and immobilized on pumice stones A. niger mycelium.The basic conditions for starch hydrolysis, securing the maximal enzyme yields, were optimized.The immobilized A. niger mycelium was able to produce the enzyme at a small loss of its activity(about 28%) during ten 24-h batches. Free mycelium produced about 1.5 times higher GODactivity than the immobilized mycelium on glucose and starch hydrolyzates. However, the latterwas more stable, reaching faster the maximum of enzyme productivity, and it could be usedmany times with almost the same efficiency. Thus, the total enzyme yields produced by immobilized mycelium were much higher than those obtained using the free one.
Biotechnologia, vol.26, 3 (1994)-.
0860-7796 ; oai:rcin.org.pl:148481 ; IChB B-22
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 25, 2020
Nov 25, 2020
52
https://rcin.org.pl./publication/183891
Edition name | Date |
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Wykorzystanie hydrolizatów skrobiowych do biosyntezy oksydazy glukozowej przez Aspergillus niger | Nov 25, 2020 |
Szczodrak, Janusz Fiedurek, Jan
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Wiater, Adrian Szczodrak, Janusz