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Utility of starch raw materials for biosynthesis of glucose oxidase by Aspergillus niger
Subtitle:Utility of starch raw materials for biosynthesis of glucose oxidase by Aspergillus niger
Creator:Fiedurek, Jan ; Szczodrak, Janusz
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:Potato starch and its hydrolyzates were used to substitute glucose carbon sources to synthesize glucose oxidase (GOD) by free and immobilized on pumice stones A. niger mycelium.The basic conditions for starch hydrolysis, securing the maximal enzyme yields, were optimized.The immobilized A. niger mycelium was able to produce the enzyme at a small loss of its activity(about 28%) during ten 24-h batches. Free mycelium produced about 1.5 times higher GODactivity than the immobilized mycelium on glucose and starch hydrolyzates. However, the latterwas more stable, reaching faster the maximum of enzyme productivity, and it could be usedmany times with almost the same efficiency. Thus, the total enzyme yields produced by immobilized mycelium were much higher than those obtained using the free one.
Relation:Biotechnologia, vol.26, 3 (1994)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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