Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Reverse- transcription polymerase chain reaction has been applied to the detection of FMDviral in samples. Total RNA was extracted with quanidinum thiocyanate-phenol-chloroform method and reversely transcribed using specific or random primers. cDNA was used as a templatefor the amplification by RT-PCR of the 424 bp of the VPl coding sequence. Random primersp(dN)e gave better results.
Biotechnologia, vol.42, 3 (1998)-.
0860-7796 ; oai:rcin.org.pl:143897 ; IChB B-38
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 18, 2020
Oct 22, 2020
88
https://rcin.org.pl./publication/179225
Edition name | Date |
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Skuteczność wykrywania RNA wirusa pryszczycy metodą PCR w zależności od starterów używanych do syntezy cDNA | Nov 18, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Paprocka, Grażyna Kęsy, Andrzej
Paprocka, Grażyna Kęsy, Andrzej
Mizak, Beata Kęsy, Andrzej Paprocka, Grażyna