Title:

Skuteczność wykrywania RNA wirusa pryszczycy metodą PCR w zależności od starterów używanych do syntezy cDNA

Subtitle:

Skuteczność wykrywania RNA wirusa pryszczycy metodą PCR w zależności od starterów używanych do syntezy cDNA

Creator:

Paprocka, Grażyna

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1998

Subject and Keywords:

biotechnology

Abstract:

Reverse- transcription polymerase chain reaction has been applied to the detection of FMDviral in samples. Total RNA was extracted with quanidinum thiocyanate-phenol-chloroform method and reversely transcribed using specific or random primers. cDNA was used as a templatefor the amplification by RT-PCR of the 424 bp of the VPl coding sequence. Random primersp(dN)e gave better results.

Relation:

Biotechnologia, vol.42, 3 (1998)-.

Volume:

42

Issue:

3

Start page:

114

End page:

120

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-38

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open