Subtitle:

Expression of Erwinia chrysanthemi genes coding pectate lyases ininfected potato tissue

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Erwinia chrysanthemi mutants, containing transcriptional fusion of one ofthe pectate lyase genes (pelA, pelB, peIC, pelD, pelE, pell, pelL, pelZ) with the reporter gene encoding p-giucuronidase activity, were studied for their ability to cause disease symptoms and to synthesise pectinases after inoculation of potatotubers. The strains affected in pell and pelL genes displayed a reduced virulenceon potato tubers demonstrating the important role of these isoenzymes in softroot disease. Analysis of the bacterial population showed an active multiplication of bacteria during the infection. Similar kinetics of growth were observedfor all mutants and for the wild type strain. Comparison of the mutants ?.nd thewild type strain showed that the pell, pelL and pelZ mutants synthesised reducedlevels of Pels. The expression ofpelA, pelE and pelZ is 5-fold higher in planta thanin in vitro. In contrast, both pell and pelL are highly (ł0-fold factor) induced inplanta, which is characteristic of the plant-inducible pectate lyases

Relation:

Biotechnologia, vol.50, 3 (2000)-.

Volume:

50

Issue:

3

Start page:

202

End page:

214

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:141418 ; IChB B-46

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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