Subtitle:

Isolated microspores as a source doubled haploids of barley

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

In vitro techniques for doubled haploids (DH) production allow for obtaining homozygous lines in a single generation. This is connected with shorterbreeding cycle of the new variety. DH lines have a potential for being used inthe selection of recombinants, stabilising of transformed lines and molecularmapping. DH lines are produced from isolated microspores through haploidembryogenesis. Microspore culture has several advantages over anther culture:it reduces the time of cultures, enables monitoring of the earliest phase of embryogenesis, allows direct development of embryos, facilitates the in vitro selection and mutation, allows for avoiding regeneration from somatic anther tissues. Moreover, microspore culture appears to be a promising tool in genetic manipulations (transformation, mutagenesis) and it can be used as a source of protoplasts and suspensions

Relation:

Biotechnologia, vol.52, 1 (2001)-.

Volume:

52

Issue:

1

Start page:

139

End page:

142

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:140018 ; IChB B-47

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty