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In vitro regeneration and transformation of rye (Secale cereale L.)
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
In vitro cultures are an integral part of plant transformation process. Genetic manipulation can be performed only on a single cell level. Therefore in vitroculture and regeneration of plants from a single cell are very important for successful transformation in vitro culture of rye is more difficult to conduct than ofothers cereals. Difficulties with in vitro regeneration of rye seems to be the mainfactor limiting the development of rye transformation systems. Genetic transformation process includes three main steps; single cell transformation, selection oftransgenic cells and regeneration of plants from single cells. Efficiency ofeach of these steps can influence the result of the transformation process. Therefore optimisation of these steps is very important.
Biotechnologia, vol.51, 4 (2000)-.
0860-7796 ; oai:rcin.org.pl:140215 ; IChB B-47
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 21, 2020
228
https://rcin.org.pl./publication/175613
Edition name | Date |
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Regeneracja i transformacja żyta (Secale cereale L.) w kulturach in vitro | Oct 2, 2020 |
Zimny, Janusz Sowa, Sławomir Menke-Milczarek, Izabela Czaplicki, Andrzej Sowa, Aleksander
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Oleszczuk, Sylwia Zimny, Janusz