Subtitle:

Molecular markers, their genetic background and some applications in plant genetics

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The presented paper gives an insight into the genetic background of molecular diversity at the DNA level. Its potential sources, classified into two maincategories depending on whether they lead to DNA sequence alternations (pointmutations, insertions, deletions, chromosomal rearrangements, mobile elementsetc.) or changes in DNA methylation pattern, are discussed in parallel to their‘molecular markers’. A general overview ofthe most important sequences responsible for genetic variability (micro-, mini-, midi- and macrosatellites; transposones and retrotransposones) is given. Special attention is paid to different typesof molecular marker systems and their most important applications. Moleculartechniques are divided into several groups depending on the enzymes they use(endonucleases, T4 DNA ligase, Taq DNA polymerase or their combinations). Finally, investigation carried out with molecular markers in somaclonal embriogenesis is discussed.

Relation:

Biotechnologia, vol.52, 1 (2001)-.

Volume:

52

Issue:

1

Start page:

9

End page:

34

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:140001 ; IChB B-47

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open