Subtitle:

Kinetic study of enzymatic hydrolysis of tannins

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Tannins are present in many plant foods. Tannase (EC 3.1.1.20) catalyses hydrolysis of ester bonds in hydrolysable gallotannins releasing glucose and gallicacid. Enzyme was produced by Penicillium chrysogenum cultured in Czapek-Doxmedium containing tannic acid. The tannic acid was used as a substrate in theenzymatic reactions. The product inhibits the enzymatic reaction. It was determined that inhibition was of the competitive type. The mathematical model(Eg. 7) and kinetic constancy describing the process were determined:Km = 0,53-10 ‘'|mol/dm3), Rm = 1,2610-3 (mol/dm^minj, Kp=2,04-10-'* (mol/dm^j.The results obtained from the model well agree with those obtained experimentally.

Relation:

Biotechnologia, vol.57, 2 (2002)-.

Volume:

57

Issue:

2

Start page:

184

End page:

192

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138091 ; IChB B-53

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open