Subtitle:

Plant Embryogenesis in vitro

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The ability of plant cells to regenerate complete organisms by somatic embryogenesis in vitro is one of the most important features of plants.Somatic embryogenesis may be defined as the development of embryos from somatic cells, haploid cells or gametopytes and has been observed in about 200 Angiosperm and Gymnosperm species. Somatic embryogenesis in vitro has been described as occuring in two general ways: direct and indirect. The process of indirect embryogenesis can be divided into two phases: 1) induction of callus mass growth and 2) development of proembryogenic cells into the embryos.The potential for somatic embryogenesis is not only genotype-specific but also strongly dependent on the developmental stage of explant and culture conditions. Embryogenic cells formation requires auxin of which 2,4-di-chloro acetic acid (2,4-D) is more commonly used, but the formation of somatic embryos occurs in auxin-free medium. Although a wide range of basal (media) has been used for induction of somatic embryogenesis, the best results were obtained with the Murashige and Skoog salts.An understanding of somatic embryogenesis process increases our ability to utilize it as the technology for mass propagation, modification and improvement of plants.

Relation:

Biotechnologia, vol.9-8, 3-2 (1990)-.

Volume:

9-8

Issue:

3-2

Start page:

52

End page:

59

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:185999 ; IChB B-06

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open