Inny tytuł:

Methods for isolation, purification and renaturation of recombinant proteins obtained in Escherichia coli bacteria

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

Escherichia coli is the most useful bacterial species applied to genetic engineering in recombinant proteins production process. The supply of many polipeptides whith potential clinical orindustrial use is often limited by their low natural availability. Overexpressed polipeptides mayeither be located in the cytoplasm and periplasm of E. coli or secreted through the cell membraneinto the growth medium. Foreign proteins can be expressed in E. coli cells directly or as fusionproteins with prokaryotic sequences. Frequently, the overexpressed proteins acumulate in thebacterial cytoplasm or periplasm in the form of insoluble inclusion bodies.This review considers isolation, purification, solubilization and renaturation of recombinantproteins from E. coli cells, whieh is still a serious methodological and technical problem.

Czasopismo/Seria/cykl:

Biotechnologia, vol.27, 4 (1994)-.

Tom:

27

Zeszyt:

4

Strona pocz.:

102

Strona końc.:

125

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:148136 ; IChB B-23

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open