Subtitle:

Stability of antisense oligonucieotides— the possibilities of their enzymatic digestion

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Nucleolytic enzymes (5 - and 3'-nucleases, exo- and endonucleases) can degrade short unmodified oligonucleotides applied as potential antisense agents. Better stability of oligonucleotidesagainst nucleolytic enzymes can be achieved by chemical modification of intemucleotide bonds.Stability of the phosphorothioate, phosphorodithioate and methylphosphonate analogues of oligonucleotides against nuclease PI, 3'-exonuclease, snake venom phosphodiesterase and other nucleases is discussed.

Relation:

Biotechnologia, vol.27, 4 (1994)-.

Volume:

27

Issue:

4

Start page:

50

End page:

54

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:148130 ; IChB B-23

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open