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RCIN and OZwRCIN projects

Object

Title: Antisense Oligonucleotides Against Inducible Nitric Oxide Synthase Reduce NO Production in RAW 264.7 Cells

Subtitle:

Antisense Oligonucleotides Against Inducible Nitric Oxide Synthase Reduce NO Production in RAW 264.7 Cells

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Description:

Zeszyt specjalny. Polish - Japan Issue

Abstract:

Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form (S-ODNs)targeted to iNOS mRNA were tested in RAW 264.7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-dependent manner. Maximalinhibitory effect was >90% while control S-ODNs were ineffective. Application of Lipofectin Reagent, which itself had no significant effect, greatly increased antisense activity. Decreased levelsof the target mRNA, as demonstrated by reverse transcriptase-polymerase chain reaction (RTPCR), suggest the RNase H mediated mechanism.

Relation:

Biotechnologia, vol.35, 4 (1996)-.

Volume:

35

Issue:

4

Start page:

173

End page:

182

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:146566 ; IChB B-31

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty

Object collections:

Last modified:

Nov 9, 2020

In our library since:

Nov 9, 2020

Number of object content downloads / hits:

52

All available object's versions:

https://rcin.org.pl./publication/182246

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