Title:

Antisense Oligonucleotides Against Inducible Nitric Oxide Synthase Reduce NO Production in RAW 264.7 Cells

Subtitle:

Antisense Oligonucleotides Against Inducible Nitric Oxide Synthase Reduce NO Production in RAW 264.7 Cells

Creator:

Bilecki, Wiktor ; Okruszek, Andrzej ; Kwinkowski, Marek ; Sanak, Marek ; Przewłocki, Ryszard

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1996

Description:

Polish - Japan Issue

Subject and Keywords:

biotechnology

Abstract:

Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form (S-ODNs)targeted to iNOS mRNA were tested in RAW 264.7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-dependent manner. Maximalinhibitory effect was >90% while control S-ODNs were ineffective. Application of Lipofectin Reagent, which itself had no significant effect, greatly increased antisense activity. Decreased levelsof the target mRNA, as demonstrated by reverse transcriptase-polymerase chain reaction (RTPCR), suggest the RNase H mediated mechanism.

Relation:

Biotechnologia, vol.35, 4 (1996)-.

Volume:

35

Issue:

4

Start page:

173

End page:

182

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-31

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open