Chachulska, Anna Maria ; Chrzanowska, Mirosława ; Flis, Bogdan ; Krzymowska, Magdalena ; Lipska-Dwuźnik, Anna ; Robaglia, Christophe ; Zagórski, Włodzimierz
Potato and tobacco cultivars transformation towards potato virus Y resistance
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
In recent years a new subgroup of necrotic potato virus Y (PVY) isolates has spread in thePolish fields. To counter this infection, the PVY resistance was introduced into selected potatoand tobaeeo cultivars. Plants were transformed by agroinfection with pROK2 derived binaryplasmids, carrying an appropriate fragment of the PVY genome (patent applied for). Kanamycinresistant plants were screened by PCR for the presence of PVY cDNA inserts, positive transformants were tested for virus resistance by inoculation with sap from infected plants, followed byobservations of disease symptoms and virus accumulation analysis. Several resistant potato andtobacco clones were identified and their phenotypes were preliminarily characterised.
Biotechnologia, vol.39, 4 (1997)-.
0860-7796 ; oai:rcin.org.pl:145338 ; IChB B-35
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 18, 2020
Oct 30, 2020
54
https://rcin.org.pl./publication/180697
Edition name | Date |
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Potato and tobacco cultivars transformation towards potato virus Y resistance | Nov 18, 2020 |
Pałucha, Andrzej Chrzanowska, Mirosława Zagórski, Włodzimierz Hulanicka, Danuta
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Doroszewska, Teresa Chachulska, Anna Maria
Sadowy, Ewa Zagórski, Włodzimierz Hulanicka, Danuta