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Potato and tobacco cultivars transformation towards potato virus Y resistance
Subtitle:Potato and tobacco cultivars transformation towards potato virus Y resistance
Creator:Chachulska, Anna Maria ; Chrzanowska, Mirosława ; Flis, Bogdan ; Krzymowska, Magdalena ; Lipska-Dwuźnik, Anna ; Robaglia, Christophe ; Zagórski, Włodzimierz
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:In recent years a new subgroup of necrotic potato virus Y (PVY) isolates has spread in thePolish fields. To counter this infection, the PVY resistance was introduced into selected potatoand tobaeeo cultivars. Plants were transformed by agroinfection with pROK2 derived binaryplasmids, carrying an appropriate fragment of the PVY genome (patent applied for). Kanamycinresistant plants were screened by PCR for the presence of PVY cDNA inserts, positive transformants were tested for virus resistance by inoculation with sap from infected plants, followed byobservations of disease symptoms and virus accumulation analysis. Several resistant potato andtobacco clones were identified and their phenotypes were preliminarily characterised.
Relation:Biotechnologia, vol.39, 4 (1997)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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