Subtitle:

Wpływ eksplantatu inicjalnego na efektywność transformacji Gerbera hybrida za pomocą Agrobacterium tumefaciens

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

An efficient method for genetic Agrobacterium tumejaciens-mediated transformation of fivecultivars Gerbera hybrida was established. The youngest leaves and shoot tips from proliferatingin vitro cultures were co-cultivated with disarmed strain LBA 4404/pBI121 carrying the chimaericgenes nptll and gus. The inoculated explants were repeatedly cultured on regeneration mediumwith kanamycin and Biotaksym. After 9-10 months from 0.04 to 0.25 independently transformedshoots from one inoculated leaf and from 0.06 to 0.65 from one inoculated shoot tip wereobtained, depending on cultivar and additional treatment. Transformed shoots accounted for 5.0to 50.0% of all regenerated shoots. Additional wounding of leaves before inoculation by squeezingand scratching off increased transformation efficiency. Better results for recalcitrant to regeneration cultivars were obtained if squeezed shoot tips were inoculated. Regenerating shoots wereselected on kanamycin, screened for gas expression with X-gluc and tested by PCR for nptlland gus genes. All together 162 transgenic shoots were obtained in cvs Amber, Boy, Ferrari,Mariola and Tamara, but only 24 relatively stable lines were established.

Relation:

Biotechnologia, vol.39, 4 (1997)-.

Volume:

39

Issue:

4

Start page:

27

End page:

37

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:145319 ; IChB B-35

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open