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Attempts at stimulating tobacco resistance to virus diseases
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Preliminary investigations were carried out on the transformation of highsusceptibility tobacco cultivars. The plants were transformed by being infectedwith Agrobacterium tumefaciens with binary PROK2 - derived plasmids carryinga PVY casette in the sense and antisense orientations and with a plasmid carrying the lettuce mosaic virus (LMV) coat protein gene. Kanamycin resistant plantswere obtained following transformation. PCR testing of a selected group of regenerants revealed the presence of a transgene. Disease symptoms were absentfrom 52% oftransgenic plants inoculated with PW^. This was confirmed by ELISAvalues.
Biotechnologia, vol.51, 4 (2000)-.
0860-7796 ; oai:rcin.org.pl:140300 ; IChB B-47
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 22, 2020
111
https://rcin.org.pl./publication/175649
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