Subtitle:

Monoclonal antibodies against isoprenoid compounds

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Monoclonal antibodies against isoprenoid compounds were obtained by invivo immunization of Balb/c mice with UQ-10 (ubiquinone-10) incorporated intocholesterol rich particles containing metylated bovine serum albumin(CRP-MBSA) in order to induce a strong immune response. The specificity of antibodies was evaluated by a modified dot-blot technique using Hybond-N +membrane (Amersham). Selected antibodies 1II-5 (lgG3), lV-3 (IgG2b) and VII-11(IgM) were capable of recognizing different isoprenoid compounds: ubiquinone,plastoquinone, solanesol, dolichol, p-sistosterol but did not react withphospholipids, triacylglycerols or fatty acids. These antibodies may be used inimmunoblotting for identifying isoprenoids (e.g. ubiquinone) in partially purified plant extracts after TLC chromatography on glass RP-18 plates (Merck).Immunodetection ofisoprenoids was performed using secondary goat anti-miceimmunoglobin antibodies conjugated with biotin, extravidine-AP and BCIP/NBTas substrate.

Relation:

Biotechnologia, vol.54, 3 (2001)-.

Volume:

54

Issue:

3

Start page:

169

End page:

177

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138885 ; IChB B-50

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty