Wanke, Małgorzata ; Porębska, Anna ; Wałajtys-Rode, Elżbieta
Monoclonal antibodies against isoprenoid compounds
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Monoclonal antibodies against isoprenoid compounds were obtained by invivo immunization of Balb/c mice with UQ-10 (ubiquinone-10) incorporated intocholesterol rich particles containing metylated bovine serum albumin(CRP-MBSA) in order to induce a strong immune response. The specificity of antibodies was evaluated by a modified dot-blot technique using Hybond-N +membrane (Amersham). Selected antibodies 1II-5 (lgG3), lV-3 (IgG2b) and VII-11(IgM) were capable of recognizing different isoprenoid compounds: ubiquinone,plastoquinone, solanesol, dolichol, p-sistosterol but did not react withphospholipids, triacylglycerols or fatty acids. These antibodies may be used inimmunoblotting for identifying isoprenoids (e.g. ubiquinone) in partially purified plant extracts after TLC chromatography on glass RP-18 plates (Merck).Immunodetection ofisoprenoids was performed using secondary goat anti-miceimmunoglobin antibodies conjugated with biotin, extravidine-AP and BCIP/NBTas substrate.
Biotechnologia, vol.54, 3 (2001)-.
0860-7796 ; oai:rcin.org.pl:138885 ; IChB B-50
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 14, 2020
130
https://rcin.org.pl./publication/174181
Edition name | Date |
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Uzyskanie przeciwciał monoklonalnych przeciwko związkom izoprenoidowym | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan