Subtitle:

Induction of genetic variation in vitro

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The effectiveness of traditional methods for inducing genetic variation hasgreatly increased with the introduction of various techniques in vitro. The newmethods of obtaining generative and somatic hybrids in vitro have resulted in agreater recombinant variation, exceeding the levels delimited hitherto by mating barriers. The potential for producing mutants has expanded due to the useof mutant somatic cells (brought in with the explant) as well as to the application of mutagens to individual cells and protoplasts, the haploid ones in particular. Two specific types of variation, i.e. somaclonal and gametoclonal variation,have proved to arise under the influence of various factors in tissue culture.However, the full application of these two types is inhibited to some extent bythe constrains on the regeneration ability of plants in culture, on the possibilityto select variants in vitro, and on the continuity of the resulting changes. Cultures in vitro also make it possible to introduce directional genetic changesthrough the application of molecular techniques.

Relation:

Biotechnologia, vol.54, 3 (2001)-.

Volume:

54

Issue:

3

Start page:

145

End page:

151

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138883 ; IChB B-50

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open