Subtitle:

Classic techniques for improvement of industrial yeast strains: Part III - A method for enucleation of Saccharomyces sp.

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficientmutants of Saccharomyces cerevisiae were enucleated after treatment withcytochalasin B.Removal of nuclei from protruding sphaeroplasts was induced bycentrifugation in a Percoll density gradient. The enucleation yield (which averaged about 80%) and the quality of the cytoplasts were best when the yeast culture had been synchronized with nocodazole before the preparation. The presence of 1 mM CaCU and ATP (10 pM) in the enucleation medium prevented theformation of fragile products or aggregation. Cytoplasts could be stored for atleast 1 day without visible deterioration.

Relation:

Biotechnologia, vol.56, 1 (2002)-.

Volume:

56

Issue:

1

Start page:

187

End page:

194

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138414 ; IChB B-52

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open