Subtitle:

Journal of Biotechnology, Computational Biology and Bionanotechnology ; Biotechnologiczne wykorzystanie gruczołu mlecznego - perspektywy manipulacji genetycznych białkami mleka

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In this article, state of art and perspectives in mammary gland biotechnology are reviewed.Recent progress in recombinant DNA technology as well in embryo manipulation and transferhas made the introduction of specific genes into the germline of animals relatively easy. Withappropriate genetic constructs, the expression of the inserted genes in transgenic animals canbe controlled in a tissue-specific and in a differentiation-specific manner. Thus, it is now possibleto consider alteration of the composition of milk produced by lactating animals in a variety ofways. There is a growing list of foreign milk proteins that have been expressed, and one canenvisage placing almost any protein gene of interest under the control of promoter of a milkprotein gene. Many human proteins of a potential pharmaceutical use may be now produced inthe mammary glands of laboratory or farm animals. Modification of milk composition can beextended not only to produce proteins of commercial value but also, by manipulation of keymetabolic enzymes, to fat, lactose, and other components of milk. Many alternations in ruminantsmilk composition, including „humanization” of cow’s milk, are planned, however, these manipulations must avait the development of totipotent embryonic cell lines (ESC) of fairn animals,cells that enable gene manipulation by homologous recombination. In spite of a great progress,many obstacles and difficulties still exist on the way to economical production of human paharmaceuticals in farm „transgenic bioreactoractors”. These dificulties are discussed in detail.

Relation:

Biotechnologia, vol.41, 2 (1998)-.

Volume:

41

Issue:

2

Start page:

33

End page:

56

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:144284 ; IChB B-37

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open