Advanced search
Advanced search
Advanced search
Advanced search
Advanced search
Antczak, Tadeusz ; Bugla, J. ; Mastalerz, M. ; Niemiec, A. ; Szeja, W. ; Ślęk, M. ; Galas, E.
Entrapment of immobilised in situ intracellular lipase of Mucor in microporous capsules
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Lipases Mucor circinelloides and Mucor racemosus immobilised in situ were closed in microporous polysacharides hydrophilic gel cross-linked using a solutionof calcium salts. In order to increase the porosity of polysacharides matrix during its cross-linking oligomer molecules of ethylene oxide (optimal Polikol1000) were incorporated in the structure of the matrix. Then the oligomer wasremoved by acetone extraction. The obtained biocatalyst preparations were tested in hydrolysis of esters and esterification of oleic acid with butanol. The hydrolysis was carried out in water saturated organic solvents medium (n-hexaneand diisopropylether). It was found that the efficiency of M. racemosus lipase immobilisation in hydrolysis of n-butyl oleate, n-butyl palmitate, n-butyl stearate,n-butyl laurate amounted to 60%. The efficiency of M. circinelloides lipase immobilisation in esterification of oleic acid with 1-butanol in hexane achieved 45%
Biotechnologia, vol.51, 4 (2000)-.
0860-7796 ; oai:rcin.org.pl:140301 ; IChB B-47
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 22, 2020
110
https://rcin.org.pl./publication/175651
Edition name | Date |
---|---|
Immobilizacja unieruchomionych in situ lipaz Mucor w mikroporowatych kapsułkach alginianowych | Oct 2, 2020 |
Sławińska, Anna Siwek, Maria
Tyczewska, Agata Bąkowska- Żywicka, Kamila
Pieczyński, Marcin Bielewicz, Dawid Dolata, Jakub Szweykowska-Kulińska, Zofia
Broniarczyk, Justyna Koczorowska, Maria Magdalena Durzyńska, Julia Warowicka, Alicja Goździcka-Józefiak, Anna