Subtitle:

Micropropagation of Arnica montana L. and sesquiterpene lactones in regenerated plants

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Plants of Arnica montana L. were micropropagated from shoot tips. Shootproliferation was obtained on Murashige and Skoog (MS) medium containingauxin (lAA 0.5 pM/1) and cytokinin (BAP, Z, TDZ). BAP (0.25 pM/l) and Z (0.5 pM/l)were most effective for proliferation and elongation ofshoots. Under these conditions, about 6 shoots per explant were obtained. Shoot proliferation was alsoachieved in the presence of TDZ (0.05 pM/1). However, when used in higherconcentration (0.2-10 pM/1), thidiazuron caused shoot vitrification. Rooting ofshoots was induced an MS medium without growth regulators. The plantletswere transplanted into pots. Phytochemical analysis showed that 4-week-oldplantlets produced sesquiterpene lactones.

Relation:

Biotechnologia, vol.51, 4 (2000)-.

Volume:

51

Issue:

4

Start page:

118

End page:

122

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:140298 ; IChB B-47

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open