Wołoszyńska, Magdalena ; Oziemlak, Aneta ; Jańska, Hanna
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Heteroplasmy is the state characterised by the presence of more than onetype of mitochondrial or chloroplast genome in one organism. Infrequent recombinations across short repeated sequences often lead to heteroplasmy inhigher plant mitochondria. Different Pbaseolus species have been examined inorder to understand the dynamics of heteroplasmy originating through recombination mediated by the 314 bp repeated sequence. Two techniques were applied to detect heteroplasmy: Southern hybridization with prolonged autoradiography and PCR amplification followed by hybridization or reamplification.In all examined genomes the four recombination forms were detected. However,these forms do not occur in the same relative amounts. Moreover, in the examined genomes different recombination forms exist at the predominant/substoichiometric level, but always only two ofthem are predominant. Based on theseresults and the previous data, we suggest that the changes in the heteroplasmicpopulation of mitochondrial molecules in plants may offer the source of geneticvariation in the course of evolution.
Biotechnologia, vol.52, 1 (2001)-.
0860-7796 ; oai:rcin.org.pl:140003 ; IChB B-47
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Oct 2, 2020
Sep 19, 2020
156
https://rcin.org.pl./publication/175461
Edition name | Date |
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Heteroplazmia u roślin | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.
Kmieć, Beata Drynda, Robert Wołoszyńska, Magdalena
Składanowski, Andrzej Cezary Bednarski, Włodzimierz Bielecki, Jacek Bielecki, Stanisław Długoński, Jerzy Dubin, Adam Fiedurek, Jan Gabryś, Halina Jańska, Hanna Kur, Józef Skorupska, Anna Szweykowska-Kulińska, Zofia Turkiewicz, Marianna Wojtaszek, Przemysław Zabża, Andrzej Podhajska, Anna Jadwiga