Bartoszewski, Grzegorz ; Szwacka, Maria ; Czarny, Magdalena ; Niemirowicz-Szczytt, Katarzyna ; Malepszy, Stefan
Journal of Biotechnology, Computational Biology and Bionanotechnology
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Plant transformation is a technology widely used in gene functional analysis and crop improvement. In this article we have attempted to sum up the studies on plant transformation carried out by the Department of Plant Genetics, Breeding and Biotechnology, Warsaw University of Life Sciences, pointing out to recent developments in this field. Efficient Agrobacterium-hased transformation protocols for cucumber and tomato were established and applied. Several traits, including fruit taste (thaumatin gene), chilling tolerance (pGT::DHN24), parthenocarpy (DefH9::iaalVl), and virus resistance (TSWV nucleoprotein gene), were modified. Transgenic cucumber lines expressing mitochondrially targeted GFP protein were developed. Sensory evaluation of fruit traits and of unintended effects of cucumber expressing thaumatin gene was made. Cucumber and tomato transformation was also applied with the aim to carry out gene functional analysis. Having introduced overexpression, silencing, and promoter gene constructs, we were able to obtain several transgenic tomato lines. Attempts have been made to set up an efficient method of sweet pepper transformation.
Biotechnologia, vol.91, 4 (2010)-.
application/pdf ; application/pdf
oai:rcin.org.pl:71072 ; IChB B-86
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
2 paź 2020
15 maj 2019
741
https://rcin.org.pl./publication/92980
Nazwa wydania | Data |
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Wybrane aspekty transformacji genetycznej ogórka, pomidora i papryki | 2 paź 2020 |
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