Subtitle:

Application of molecular biology methods for blood testing

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In this paper a simple, rapid and reliable test for sex diagnosis has been described. The test is basedon detection of alphoid satellite sequences in undigested genomic DNA using the polymerase chain reaction (PCR) and dried blood specimens serving as the source of DNA. When female DNA was used as atemplate for the reaction, only the expected X-chromosome-specific fragment was detected, while withmale DNA both X-specific and Y-specific-fragments were detected. The Y-chromosome-specific fragment was further characterized by restriction enzyme analysis. The Y fragment was detectable when DNAobtained from an equivalent of 5 n\ of spotted blood was used in the reaction. This test may find applications in sexing of human DNA for forensic purposes, in following host versus graft cells in patients withbone marrow transplants from the opposite sex or in searching for XO/XY mosaicism in patients withTurner syndrome.

Relation:

Biotechnologia, vol.13-14, 3-4 (1991)-.

Volume:

13-14

Issue:

3-4

Start page:

127

End page:

130

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:180511

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open