Inny tytuł:

Gene cloning using subtractive hybridization

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

Genes expressed in temporally, environmentally or tissue specific way can be cloned using subtractivehybridization. It involves hybridization of single stranded cDNA from one cell type with mollar excess ofpolyA+RNAfrom another cell type. Unhybridized, cell specific cDNA is subsequently used or as subtractiveprobe or as material for construction of subtractive cDNA library. Modifications of this basic strategy arealso discussed.

Czasopismo/Seria/cykl:

Biotechnologia, vol.17, 2 (1992)-.

Tom:

17

Zeszyt:

2

Strona pocz.:

26

Strona końc.:

35

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:163606 ; IChB B-13

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open