Gene cloning using subtractive hybridization
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Genes expressed in temporally, environmentally or tissue specific way can be cloned using subtractivehybridization. It involves hybridization of single stranded cDNA from one cell type with mollar excess ofpolyA+RNAfrom another cell type. Unhybridized, cell specific cDNA is subsequently used or as subtractiveprobe or as material for construction of subtractive cDNA library. Modifications of this basic strategy arealso discussed.
Biotechnologia, vol.17, 2 (1992)-.
0860-7796 ; oai:rcin.org.pl:163606 ; IChB B-13
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Feb 26, 2021
Feb 26, 2021
335
https://rcin.org.pl./publication/197358
Edition name | Date |
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Klonowanie genów metodą hybrydyzacji różnicowej | Feb 26, 2021 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.
Missol, Ewa Sochanik, Aleksander Szala, Stanisław