Subtitle:

Mechanical damage of animal cells in bioreactors

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Fluid-mechanical damages of shear sensitive animal cells due to forces generated by mediaagitation and aeration are reviewed. In anchorage-dependent cultures grown in a stirred bioreactor the cell damage is caused by small turbulent eddies of size of the microcarrier beads andby collision between microcarriers and against the impeller and the stationary parts of bioreactor.In the freely suspended cells grown in stirred or air-lift reactors the cell damage is due mainlyto air bubble breakup. The mechanical damages can be limited by an increase of kinematicviscosity of fluid and reduction of the local energy dissipation rate. Biological aspects of shearstress are also discussed.

Relation:

Biotechnologia, vol.22, 3 (1993)-.

Volume:

22

Issue:

3

Start page:

166

End page:

183

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:154171 ; IChB B-18

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open