Inny tytuł:

Age-related Fragmentation of Mitochondrial DNA Associated with Oxygen Damage

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Opis:

Polish - Japan Issue

Abstrakt:

In normal human hearts, a progressive age-related fragmentation of mitochondrial (mt) DNAinto various-sized deleted (A) mtDNA up to 358 types was documented by a novel total-detectionsystem for deletions. The AmtDNA lacking replication origin(s), minicircles, accumulated up to280 types out of the 358, suggesting a yet unknown replication mechanism in human. Wild-typemtDNA decreased linearly down to 11% of the total with age negatively correlated with AmtDNAand oxidized nucleoside, 8-hydroxy-deoxyguanosine. A remarkable mirror image observed inAmtDNA size distribution as well implies that random hydroxyl-radical attacks resulted in doublestrand break and rejoining of mtDNA as a preferable mechanism to form various AmtDNA ofclosed circular duplex. In the patients with mitochondrial cardiomyopathy, similar fragmentationand oxidative damage in mtDNA was documented at their age 7 to 19 equivalent to the normalsubjects of age over 80. Exposure of a cultured cell line under oxygen stress, 95% oxygen, coldmimic these changes in mtDNA* within 3 days leading an apoptotic cell death, whereas mtDNAlacking cells are relatively immune. These facts support the ‘redox mechanism of ageing’.

Czasopismo/Seria/cykl:

Biotechnologia, vol.35, 4 (1996)-.

Tom:

35

Zeszyt:

4

Strona pocz.:

208

Strona końc.:

222

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:146571 ; IChB B-31

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open