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Murakami, Akira ; Nakaura, Misuzu ; Uematsu, Hiroki ; Fujimura, Naoko ; Iwase, Reiko ; Yamaoka, Tetsuji
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Zeszyt specjalny. Polish - Japan Issue
In order to evaluate the antisense effects in biological systems, the interaction of antisensemolecules with biological substances such as DNAs and human serum proteins was studied byfluorescence depolarization spectroscopy. When an antisense oligonucleotide anchoring fluorescein (F-AS) was mixed with DNAs or proteins, the anisotropy value changed, indicating thatantisense oligonucleotides appreciably interact with DNAs and serum proteins, and that suchinteraction may play important roles on antisense gene regulation.
Biotechnologia, vol.35, 4 (1996)-.
0860-7796 ; oai:rcin.org.pl:146564 ; IChB B-31
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Nov 9, 2020
Nov 9, 2020
56
https://rcin.org.pl./publication/182242
Edition name | Date |
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Interaction of Antisense Oligonucleotides with DNA and Proteins Studied by Fluorescence Polarization Spectroscopy | Nov 9, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.