Subtitle:

Wykorzystanie linii komórkowych i metod synchronizacji cyklu komórkowego w klonowaniu ssaków

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Studies aimed at improving the effectiveness of cloning by nuclear transfer have shown thatproper development of the majority of reconstituted embryos is secured by G1 phase of thedonor nucleus or by using “universal recipients” i.e. enucleated pre-activated oocytes. Donornuclei for cloning may be derived from cell lines of embryonic stem cells (mouse), embryoniccells after short in vitro culture (sheep, pig, cattle) or even from fetal cells or adult cells afterin vitro culture and inducing quiescence in their nuclei (sheep).The use of fetal cells for transgenesis in vitro and the production of transgenic sheep afternuclear transfer from these cells opens the way to profitable technology of cloning transgenicfarm animals.

Relation:

Biotechnologia, vol.41, 2 (1998)-.

Volume:

41

Issue:

2

Start page:

86

End page:

100

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:144323 ; IChB B-37

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open