Subtitle:

Microspore culture of winter oilseed rape (Brassica napus L.) in conjunction with other in vitro technologies

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Microspore culture in conjuction with other technologies such as selection,mutagenesis and transformation has been used for the production of novel genotypes of Brassica napus L. for crop improvement. The example ofin vitro selection of microspore - derived embryos includes: a) ploidy level, b) seed oil composition (for example: high level of erucic acid), c) genotypes with restorer genefor CMS-ogiira system (by means of isozyme marker PGl-2), d) herbicide resistant forms. Efficiency of microspore mutagnesis has been done by the treatment of freshly isolated microspores with UV and MNU. Direct delivery of foreign gene to the microspores (microprojectile bombardment) combined withthe use of Agrobacterium tumefaciens to microspore derived embryos seems tobe a promising way of oilseed rape transformation.

Relation:

Biotechnologia, vol.52, 1 (2001)-.

Volume:

52

Issue:

1

Start page:

81

End page:

86

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:140009 ; IChB B-47

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open