Subtitle:

Glutathione Transferases and Serine Proteases: From Probing Mechanism of Enzyme Catalysis by Rational Protein Engineering to Evolutionary Design of Enzyme Function

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Research of the past decade has demonstrated that the use of enzymes andwhole-cell biocatalysts is environmentally friendly, economical and surprisingly,faces few barriers when applied in organic syntheses. In nature, enzymesevolved to function within a living system and may not exhibit features desirable for large-scale in vitro syntheses. Thus, protein engineering has the potential to dramatically enhance enzyme performance in a wide variety of unusual -but technologically important - environments. Site-directed, cassette-mutagenesis and construction of chimeric enzymes commonly used in rational protein engineering are important strategies which reveal the structure-functionrelationship of a given enzyme. Notably, construction of hybrid enzymes has become increasingly important in the rational design of novel biocatalysts withdesired properties and activities. However, “irrational” protein design based onrandom mutagenesis technologies or the combination with directed evolutionapproaches has truly revolutionized the generation offunctional biological molecules.

Relation:

Biotechnologia, vol.58, 3 (2002)-.

Volume:

58

Issue:

3

Start page:

53

End page:

60

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:137870 ; IChB B-54

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty