Metadata language
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:Research of the past decade has demonstrated that the use of enzymes andwhole-cell biocatalysts is environmentally friendly, economical and surprisingly,faces few barriers when applied in organic syntheses. In nature, enzymesevolved to function within a living system and may not exhibit features desirable for large-scale in vitro syntheses. Thus, protein engineering has the potential to dramatically enhance enzyme performance in a wide variety of unusual -but technologically important - environments. Site-directed, cassette-mutagenesis and construction of chimeric enzymes commonly used in rational protein engineering are important strategies which reveal the structure-functionrelationship of a given enzyme. Notably, construction of hybrid enzymes has become increasingly important in the rational design of novel biocatalysts withdesired properties and activities. However, “irrational” protein design based onrandom mutagenesis technologies or the combination with directed evolutionapproaches has truly revolutionized the generation offunctional biological molecules.
Relation:Biotechnologia, vol.58, 3 (2002)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
Projects co-financed by: Access: