Subtitle:

The transgenes are expressed with different level in plants

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Variable expression for the same transgene construct has been documentedin various plant species, regardless of the type of transgene. This phenomenonand the factors influencing it are reviewed. A variability in the transgene expression level was found to exist between and/or within independently derivedlines, different lines of clonal replicates, siblings ofthe same line, a single plant,a single leaf as well as the same cell. Both the transgene dependent and the recipient dependent factors have been proposed to account for this phenomenon,the transgene dependent factors of which include transgene construct fidelity,T-DNA integration pattern, T-DNA copy number, promoter activity and the effect of nuclear matrix attachment region. In addition, different forms of epigenetic, homology-dependent gene silencing also contribute to the unstable expression ofthe identical transgene. The recipient dependent factors include position effect, ploidy level, genetic background, homozygosity, and developmental stage. Furthermore, environmental factors such as light intensity, temperature, field growth conditions and the season have been shown to modulate thelevels of transgene expression. The approaches to stabilize the transgene expression were also discussed.

Relation:

Biotechnologia, vol.61, 2 (2003)-.

Volume:

61

Issue:

2

Start page:

236

End page:

260

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:135752 ; IChB B-57

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open