Inny tytuł:

Selected issues of non-conventional enzymology Part I - Characterisation of enzymatic processes carried out in amedium with subcritically low water content

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Opis:

Selected issues of non-conventional enzymology

Abstrakt:

Enzymology of non-aqueous media, also termed a non-conventionalenzymology, is a discipline focused on chemical reactions catalysed by enzymesin the media other than water. However, such environments always contain certain amount ofwater, either dissolved in a solvent or enzyme-bound. The latterfraction of water is called an essential one, and it is crucial for the non-aqueousenzymology, since it stabilises the conformation of protein molecule and determines its enzymatic activity. The paper presents structure, function, and methods of assays of the enzyme-bound water together with other water forms thatcan also be found in reaction milieu. The methods of regulation of enzymaticactivity in non-aqueous media by using compounds capable of associating withpolar atoms of amino acid residues involved in catalysis, and thus positively influencing activity of enzymes, have also been discussed. The factors giving riseto a decrease in catalytic activity of enzymes under non-aqueous conditions,and methods of prevention against this phenomenon have also been analysed.

Czasopismo/Seria/cykl:

Biotechnologia, vol.62, 3 (2003)-.

Tom:

62

Zeszyt:

3

Strona pocz.:

124

Strona końc.:

138

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:133288 ; IChB B-58

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open