Inny tytuł:

Influence of long-time continuous wine fermentation on morphological changes of yeast immobilized on foam glass

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Opis:

prace eksperymentalne

Abstrakt:

The aim of this work was to study the influence of long-time continuouswine fermentation on yeast morphology. The process of fermentation was carried out in medium of high sugar concentration (about 320 g/dm^), for 3,5months, at 22°C in 4 columns fermentor (4 series). In this experiment, wineyeast Saccharomyces bayanus strain S.O./1AD from the Collection of Pure Culturesof the Department of Food Biotechnology and Microbiology of Warsaw Agriculture University was used. Yeast was immobilized on foam glass. In this study,before and after immobilization (after finished fermentation) the cells were examined by light microscopy, scanning electron microscopy and computer imageanalysis. It was stated that yeast after 3,5 months of continuous fermentationwas bigger and showed morphological and intracellular changes in comparisonwith cells before this process. Before immobilization yeast was ovoid, single orbudding cells. Various shapes of cells were observed after immobilization: elongated, in the shape of “pear”, very big round and “pseudomycelia”. In “pseudomycelium”, the cells remained firmly connected by their cell walls and cytoplasm. In some cells, degeneration of cytoplasmic content was observed, too.These cells contained “granularity”. The results show that long-time, continuous fermentation in high sugar medium causes negative changes ofyeast cells.

Czasopismo/Seria/cykl:

Biotechnologia, vol.63, 4 (2003)-.

Tom:

63

Zeszyt:

4

Strona pocz.:

167

Strona końc.:

181

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:129802 ; IChB B-59

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open