Subtitle:

Comparative in vitro study on the adhesion of probiotic and pathogenic bacteria to different human intestinal cell lines

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

The ability of four strains of Lactobacillus sp. two strains of Bifidobacterium sp. and one strain of Listeria monocytogenes to adhere to human intestinal cell lines Caco-2, HT-29 and Int 407 was examined. Well-developed monolayers of intestinal cells were obtained when initial concentration of Caco-2 cells was 1 X lO'^/cm^, HT-29 cells 4.2 x lO'^/cm^, and Int 407 cells 2 x lO'^/cm^. The appropriate fetal bovine serum additions for Caco-2, HT-29 and Int 407 were 20%, 10% and 10%, respectively. The reduction of serum addition decreased intestinal cell density and prolonged monolayer development. The highest cell densities in epithelial monolayer were obtained in the Int 407 cell cultures. The yield of bacterial adhesion was strain - dependent. Significant differences were also observed in bacteria adhesion to individual intestinal cell lines. The best adhesion ability to Caco-2 exhibited Lactobacillus rhamnosus GG and Bifidobacterium bifidum. The highest adhesion to HT-29 line demonstrated B. bifidum and Lactobacillus acidophilus LCl. The adhesion of bacteria to Int 407 was much lower. Significant effect on bacteria adhesion has their cell density being in contact with intestinal monolayer. The adherence of Listeria monocytogenes to Caco-2 and HT-29 was very low in the range of 0.2% and 6.0%, respectively.

Relation:

Biotechnologia, vol.69, 2 (2005)-.

Volume:

69

Issue:

2

Start page:

215

End page:

233

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:111552 ; IChB B-65

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty