Subtitle:

Mathematic’s models in metabolic engineering

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Metabolic engineering is an integrating methodology of analysis and synthesis for improvement of flux distribution of metabolic pathways. It has two main aspects: modeling and analysis of metabolic networks to establish strategies for pathway engineering and actual molecular level engineering the pathway. Mathematical modeling is one of the key methodologies of metabolic engineering. The review presents the currently used metabolic modeling approaches. Metabolite balancing is the basis for analysis of metabolic flux and cell capability to form a targeted product. The use of isotope - labeled substrates with nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GCMS) analyses of intracellular and extracellular metabolites enables determination of metabolic flux distribution. Metabolic Control Analysis (MCA) is a theoretical framework for investigation of control mechanism of metabolic network to identify key parameters influencing productivity. Kinetics models present a more detailed approach to simulate metabolic net behavior. Linear approximation of kinetic model, the so called (log)linear kinetics, is useful for modeling spatiotemporal variations of the net. The including of genetic regulation to metabolic models, the next step in the development of metabolism models, needs new methods of experimental approaches and mathematic and computational resources.

Relation:

Biotechnologia, vol.69, 2 (2005)-.

Volume:

69

Issue:

2

Start page:

7

End page:

31

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:111216 ; IChB B-65

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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