Optimalization and validation of ADSRRS-fingerprinting
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Macrorestriction analysis of genomic DNA followed by pulsed-field gel electrophoresis (REA-PFGE) has become the “gold standard” for molecular typing. In recent years, some alternative techniques have been successfully applied. However, there is still a need for a new method which may appear to be less complex, cheaper, and have a power of discrimination at least similar to that of REA-PFGE. Recently, we showed the performance and convenience of a novel assay, based on the fingerprinting of bacterial genomes by amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting), for its potential usefulness in epidemiological investigations.
Biotechnologia, vol.77, 2 (2007)-.
0860-7796 ; oai:rcin.org.pl:87118
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Dec 6, 2019
379
https://rcin.org.pl./publication/113503
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Handschuh, Luiza Lewandowski, Krzysztof Kaźmierczak, Maciej Komarnicki, Mieczysław Figlerowicz, Marek