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Optimization of transduction of chosen cell lines using adenoviral vectors of the first generation
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Efficacy of adenoviral vectors (AdV) was checked by transduction of six different cell lines (COS-7, HUVEC, HMEC-1, NIH 3T3, HaCaT, and B16(F10)) with the vectors containing reporter gene p-galactosidase (Adp-gal). We optimised the adsorption time and dose of Adp-gal. The transduction with efficacy of up to 100% was obtained for the doses 10-100 lU/cell. In order to examine the effect of transduction procedure on biology of the cells, we measured the production of major proinflammatory cytokines. In several cell lines (HMEC-1, HUVEC, HaCaT), we found the induction of IL-6 synthesis and decrease in the cell viability, particularly in the case of the highest Adp-gal dose. However, the treatment with adenoviral vectors did not induce TNF generation and did not modify proliferation of cells.
Biotechnologia, vol.78, 3 (2007)-.
oai:rcin.org.pl:78588 ; 0860-7796 ; IChB B-74
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Aug 16, 2019
768
https://rcin.org.pl./publication/102561
Edition name | Date |
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Optymalizacja warunków transdukcji wybranych linii komórkowych przy użyciu wektorów adenowirusowych pierwszej generacji | Oct 2, 2020 |
Stopa, Magdalena Dulak, Józef Józkowicz, Alicja
Józkowicz, Alicja Dulak, Józef
Rutkowski, Andrzej Jaźwa, Agnieszka Józkowicz, Alicja Dulak, Józef
Kucharzewska, Paulina Zagórska, Anna Leja, Justyna Jaźwa, Agnieszka Gozdecka, Małgorzata Józkowicz, Alicja Dulak, Józef
Gołda, Sławomir Kucharzewska, Paulina Cisowski, Jarosław Florczyk, Urszula Zagórska, Anna Jaźwa, Agnieszka Łoboda, Agnieszka Józkowicz, Alicja Dulak, Józef