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Optimization of transduction of chosen cell lines using adenoviral vectors of the first generation
Subtitle:Optimization of transduction of chosen cell lines using adenoviral vectors of the first generation
Creator:Stopa, Magdalena ; Dulak, Józef ; Józkowicz, Alicja
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:Efficacy of adenoviral vectors (AdV) was checked by transduction of six different cell lines (COS-7, HUVEC, HMEC-1, NIH 3T3, HaCaT, and B16(F10)) with the vectors containing reporter gene p-galactosidase (Adp-gal). We optimised the adsorption time and dose of Adp-gal. The transduction with efficacy of up to 100% was obtained for the doses 10-100 lU/cell. In order to examine the effect of transduction procedure on biology of the cells, we measured the production of major proinflammatory cytokines. In several cell lines (HMEC-1, HUVEC, HaCaT), we found the induction of IL-6 synthesis and decrease in the cell viability, particularly in the case of the highest Adp-gal dose. However, the treatment with adenoviral vectors did not induce TNF generation and did not modify proliferation of cells.
Relation:Biotechnologia, vol.78, 3 (2007)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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