Gołda, Sławomir ; Kucharzewska, Paulina ; Cisowski, Jarosław ; Florczyk, Urszula ; Zagórska, Anna ; Jaźwa, Agnieszka ; Łoboda, Agnieszka ; Józkowicz, Alicja ; Dulak, Józef
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Regulation of gene expression in gene therapy is crucial for obtaining the therapeutic effects, thanks to limitation of transgene activity to the selected cells in a given time. In this paper we have focused on plasmid expression systems regulated by doxycycline or hypoxia. We have described in details the structure, regulatory elements and biological applications of 1) the modified, commercially available Tet-On system, expressing doxycycline-controlled P-galactosidase and 2) hypoxia-activated FGF-4A^GF expression plasmid containing the hypoxia responsive sequence. The presented expression systems can also be used in viral vectors, enabling not only regulated, but also high and long-term expression of transgenes.
Biotechnologia, vol.78, 3 (2007)-.
0860-7796 ; oai:rcin.org.pl:76141 ; IChB B-74
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
2 paź 2020
27 lip 2019
1955
https://rcin.org.pl./publication/100057
Nazwa wydania | Data |
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Regulacja ekspresji genów w wektorach plazmidowych: system zależny od doksycykliny i regulowany przez niedotlenienie | 2 paź 2020 |
Kucharzewska, Paulina Zagórska, Anna Leja, Justyna Jaźwa, Agnieszka Gozdecka, Małgorzata Józkowicz, Alicja Dulak, Józef
Rutkowski, Andrzej Jaźwa, Agnieszka Józkowicz, Alicja Dulak, Józef
Józkowicz, Alicja Dulak, Józef
Stopa, Magdalena Dulak, Józef Józkowicz, Alicja
Stopa, Magdalena Dulak, Józef Józkowicz, Alicja