Subtitle:

L-(1—>3)-Glucans from cell wall of Laetiporus sulphureus (Bull.:Fr.) Murrill - isolation, characteristics and application for induction of mutanase synthesis

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Description:

prace eksperymentalne

Abstract:

Five different methods described in the literature were used for the isolation of a-(l->3)-glucans from the cell wall of fruiting bodies of Laetiporus sulphureus (Bull.iFr.) Murrill, and their comparative analysis was performed. The separated fungal biopolymers were well-characterized in respect of their structure and some physicochemical properties. Structural analyses, i.e., Fou- rier-transform infra-red (FT-IR) spectroscopy, 'FI nuclear magnetic resonance (NMR) spectroscopy and specific rotation, revealed that the alkali-soluble wall fraction from this basidiomycetous fungus contained about 56% of (1—>3)-linked a-glucans. Four out of five a-(l—>3)-glucans isolated by different methods from the mycelium of the polypore fungus L. sulphureus induced higher activity of fungal and bacterial mutanase than those obtained on mutan. Therefore, the a-(l—>3)-glucans from fruiting bodies ofL sulphureus can be used as a new alternative to streptococcal mutan, which so far has been known as the best inducer of mutanase production.

Relation:

Biotechnologia, vol.81, 2 (2008)-.

Volume:

81

Issue:

2

Start page:

174

End page:

189

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:73915 ; IChB B-76

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open