Type of object:

Journal/Article

Abstract:

A histochemical study was carried out on the activity of the dehydrogenases linked with coenzyme II (diaphorase of coenzyme II, isocitric and glucose-6-phosphatase dehydrogenase) and with coenzyme I (isocitric and alfa-glycerophosphatase dehydrogenase) in neuroglia cultivated in vitro.Glucose-6-phosphate ,alfa-glycerophosphate and isocitric dehydrogenase linked with coenzyme II showed the greatest intensity of the enzymatic reaction; less intensive reactions were obtained with isocitric dehydrogenase linked with coenzyme I; and least intensive with diaphorase of coenzyme II. Astrocytes were characterized by uniform distribution of the formazan granules in the cytoplasm and cel processes already in the earliest phases of growth of the cultures. In oligodendrocytes enzymatic activity appeared later and was localized only in the perinuclear zone of protoplasm at first, and after 10 —11days of growth in the processes. Glucose-6-phosphate dehydrogenase activity was the first to appear in the oligodendroglia, followed by the remaining dehydrogenase activities. The high activities of the studied dehydrogenases, which represent the enzymatic systems involved in the aerobic and anaerobic metabolism of glucose, indicate intensive metabolic processes in growing glial tissue, in contrast to the low level of metabolism in glial tissue in vivo.

Relation:

Polish Medical Journal

Volume:

5

Issue:

6

Start page:

1405

End page:

1417

Detailed Resource Type:

Article

Resource Identifier:

oai:rcin.org.pl:70605

Language:

eng

Language of abstract:

eng

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open